rabbit anti caix  (Proteintech)

Journal: Scientific reports

Article Title: Pharmacological inhibition of hypoxia induced acidosis employing a CAIX inhibitor sensitizes gemcitabine resistant PDAC cells.

doi: 10.1038/s41598-025-93388-5

Figure Lengend Snippet: Fig. 2. Expression of HIF-1α and CAIX in MIA PaCa-2 and PANC-1 post MZM treatment. (A) Basal levels of mRNA transcript for HIF-1α and CAIX in MIA PaCa-2 and PANC-1 cells at 3, 18 and 24 h under hypoxia and normoxia. (B) Basal expression of HIF-1α and CAIX protein in MIA PaCa-2 and PANC-1 cells at 24 and 48 h. (C, D) Expression of HIF-1α and CAIX in MIA PaCa-2 cells treated with MZM at increasing concentrations (0.1–3 mM) for 24 and 48 h under hypoxia and normoxia. (E, F) Expression of HIF-1α and CAIX in PANC-1 cells treated with MZM at increasing concentrations (0.1–3 mM) for 24 and 48 h under hypoxia and normoxia.

Article Snippet: The membranes were incubated with various primary antibodies against different proteins viz, HIF-1α (#610658, BD Biosciences, England, UK), CAIX (#5649, Cell signaling, MA, USA), Ki-67 (#ab15580, Abcam, MA, USA), VEGF (#50661, Cell Signaling, MA, USA), Cyclin D1 (#2922, Cell Signaling, MA, USA) and GAPDH (#MA5-15738, Invitrogen, Canada) overnight followed by 3 washes with PBST.

Techniques: Expressing

Journal: Scientific reports

Article Title: Pharmacological inhibition of hypoxia induced acidosis employing a CAIX inhibitor sensitizes gemcitabine resistant PDAC cells.

doi: 10.1038/s41598-025-93388-5

Figure Lengend Snippet: Fig. 3. Inhibition of HIF-1α and CAIX in PDAC cells. (A–D) Under hypoxia, mRNA transcripts levels of HIF-1α and CAIX in MIA PaCa-2 (A and B) and PANC-1 (C and D) post treatment with MZM alone or in combination with GEM. The relative expression of genes was calculated with the 2–∆∆Ct method, using β- actin as housekeeping gene for normalization. Statistical differences between the groups were determined by one-way ANOVA and post hoc multiple variances using Tukey test. The statistically significant difference is represented as *p < 0.05, **p < 0.01 and ***p < 0.001 for control v/s specific group. (E–H) Western blot showing HIF-1α and CAIX protein expression levels in MIA PaCa-2 (E and F) and PANC-1 (G and H) post treatment with MZM alone or in combination with GEM (400 nM) at 24 and 48 h under normoxia (21% O2), and hypoxia condition (1% O2). Numbers on top indicate fold difference in expression which was determined after densitometry analysis using Image J software. β-actin was used as loading control.

Article Snippet: The membranes were incubated with various primary antibodies against different proteins viz, HIF-1α (#610658, BD Biosciences, England, UK), CAIX (#5649, Cell signaling, MA, USA), Ki-67 (#ab15580, Abcam, MA, USA), VEGF (#50661, Cell Signaling, MA, USA), Cyclin D1 (#2922, Cell Signaling, MA, USA) and GAPDH (#MA5-15738, Invitrogen, Canada) overnight followed by 3 washes with PBST.

Techniques: Inhibition, Expressing, Control, Western Blot, Software